Zach Tyler Eisen

Lucas Nickle (voice)

Julia Roberts

Hova (voice)

Meryl Streep

Queen (voice)

Nicolas Cage

Zoc (voice)

Paul Giamatti

Stan Beals (voice)

Bruce Campbell

Fugax (voice)

Regina King

Kreela (voice)

Lily Tomlin

Mommo (voice)

Larry Miller

Fred Nickle (voice)

Cheri Oteri

Doreen Nickle (voice)

Ricardo Montalban

Head of Council (voice)

Myles Jeffrey

Steve (voice)

Creagen Dow

Mullet Boy (voice)

Frank Welker

Spindle / Frog / Caterpillar (voice)

Mark DeCarlo

Fly (voice)

Jake T. Austin

Nicky (voice)

Allison Mack

Tiffany Nickle (voice)

S. Scott Bullock

Glow Worm / Wasp Survivor (voice)

Paul Greenberg

Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice)

Rob Paulsen

Beetle (voice)

Nicole Sullivan

Ant #9 (voice)

Don Frye

Soldier Ant (voice)

Larry Cedar

Ant #8 (voice)

Paul Greenberg

Sleeping Ant #1 / Head Lice #1 / Head Lice #2 (voice)

David Kaye

Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice)

Alfred Jackson

Slacker Ant (voice)

Ken Mitchroney

Ant #17 / Guard Ant #2 / Wasp #8 (voice)

Denzel Whitaker

Albert (voice)

Casey Masamitsu

Shigeko (voice)

Bryan Fabian

Fernando (voice)

Jake Goldberg

Helmet Boy / Nob / Red Teammate #1 (voice)

Keith Alcorn

Ant #18 / Pupa / Soldier Ant #3 (voice)

John A. Davis

Ant #19 (voice)

Tom Kenny

Drone Ant / Ant #2 / Ant #6 (voice)

Sarah Mensinga

Mother Ant (voice)

Nika Futterman

Ant #1 / Ant #7 (voice)

Scott Holst

Ant #3 (voice)

Susan Silo

Ant #4 (voice)

Paul Rugg

Ant #5 (voice)

Candi Milo

Nurse Ant #3 (voice)

Nissa Alcorn

Billo (voice)

Pat Fraley

Ant Council #1 (voice)

Tyler James Williams

Blue Teammate #1 (voice)

Jared Goldsmith

Blue Teammate #2 / Red Teammate #2 (voice)

Austin Majors

Blue Teammate #3 (voice)

Jaishon Fisher

Blue Teammate #4 (voice)

Aaron Michael Drozin

Blue Teammate #5 (voice)

Max Burkholder

Blue Teammate #6 (voice)

Sam Green

Red Teammate #3 (voice)

Colin Ford

Red Teammate #4 (voice)

Kendall Ryan Sanders

Red Teammate #5 (voice)

Shane Baumel

Red Teammate #6 (voice)

Neil Ross

Wasp #1 / Wasp #5 (voice)

Bob Joles

Wasp #2 (voice)

Wally Wingert

Wasp #3 (voice)

Leon Morenzie

Wasp #4 (voice)

Jonathan David Cook

Wasp #6 (voice)

Sean Donnellan

Soldier Ant #1 (voice)

Tress MacNeille

Old Council Ant (voice)

Richard Green

Wasp Leader (voice)

Clive Robertson

Hova's Wasp (voice)

Vernee Watson-Johnson

Head Nurse (voice)